The PolyEnz™ Process

This process produces pre-stabilised enzymes which can then be immobilised onto or into a variety of matrices for the production of stabilised biocatalysts for production of pharmaceuticals, chemical synthesis, biotransformations and bioremediation.

The process has demonstrated on a solid activated particulate support, using an oxidoreductase enzyme as the immobilised biocatalyst.

Significant improvements of the shelf-stability of the immobilised biocatalyst have been recorded - Figure 1.

The PolyEnz™ Process also changes the microenvironment of the immobilised biocatalyst, which can lead to higher enzyme activity levels - Figure 2. The effect of this would be to improve production and potentially to improve efficiency of catalysis.

It is well known the microenvironment of enzymes often has a significant part to play in activity, stability and catalytic properties.

Such microenvironment manipulations are at the heart of AET expertise and are likely to prove crucial to the performance and stability of PolyEnz™ immobilised enzymes.

Figure 3 shows the effect of the PolyEnz™ Process on the operational stability of an oxidoreductase biocatalyst at an elevated temperature of 50°C, compared to the same biocatalyst immobilised in its native form. The improvement of activity retention is clearly apparent, leading to improved productivity and a reduction in costs overall.