Stabilisation of Proteins in Solution

The solution stabilisation protocols developed at Leeds appear to be generic in conferring thermal, chemical and proteolytic stability to a wide range of different proteins.

The protocols developed use combinations of soluble polymers and additives incorporated into the buffers used for protein stabilisation.

Particular Features Include:

  • Extended Shelf Life of Proteins in Solution
  • Promotion of Operational Stability
  • Protection Against Dilution Inactivation
  • Protection Against Proteolytic Degradation

 

Proteins Stabilised in Solution

Enzyme Length of
Stability
Storage
Temperature
(°C)
Acetylcholine Esterase 180 mins 55
Alcohol Oxidase 30 mins 56
Alkaline Phosphate (Bacterial) 476 days 4
Anti-TSH-HRP Conjugate 240 mins 58
Bovine Alkaline Phosphate 60 mins 62
Catalase 160 60
Creatinase 5 days 37
Creatininase 5 days 37
Cutinase 180 mins 88
Glucose dehydrogenase 21 days 37
Glucose Oxidase 246 days 22
Glycerol kinase 120 mins 52
Glycerol phosphate oxidase 330 mins 40
Goat anti-human IgA Alkaline Phosphatase 180 mins 55
Horseradish Peroxidase-4 180 days 40
Horseradish Peroxidase-4 24 hours 69
Lactate Dehydrogenase 5 days 37
L-Glutamate Dehydrogenase 100 days 45
Lipase 14 days 25
Luciferase 50 mins 45
Penicillin G Acylase 20 mins 60.4
Rabbit anti-DNP HRP Conjugate 24 hours 58
Rabbit anti-Human IgG-HRP Conjugate 221 days 25
Recombinant Horseradish Peroxidase 80 days 50
Sarcosine Oxidase 5 days 37
Savinase 28 days 40
Savinase 21 days 50
T4-HRP Conjugate 230 mins 67
Thermophilic Glutamate Dehydrogenase 30 days 40
Trypsin 21 days 50